Members‎ > ‎

Professor David Lamb DSc FRSB FRSC

Publications

2017

Lemaire B, Karchner SI, Goldstone JV, Lamb DC, Drazen JC, Rees JF, Hahn ME, Stegeman JJ. Molecular adaptation to high pressure in cytochrome P450 1A and aryl hydrocarbon receptor systems of the deep-sea fish Coryphaenoides armatus. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 2017, EPub ahead of print.

2016

Lemaire B, Kubota A, O'Meara CM, Lamb DC, Tanguay RL, Goldstone JV, Stegeman JJ. Cytochrome P450 20A1 in zebrafish: cloning, regulation and potential involvement in hyperactivity disorders. Toxicology and Applied Pharmacology. 2016, EPub ahead of print.

Goldstone JV, Sundaramoorthy M, Zhao B, Waterman MR, Stegeman JJ, Lamb DC. Genetic and structural analyses of cytochrome P450 hydroxylases in sex hormone biosynthesis: sequential origin and subsequent coevolution. Molecular Phylogenetics and Evolution. 2016, 94 Part B, 676-687.

2015

Hargrove TY, Wawrzak Z, Lamb DC, Guengerich FP, Lepesheva GI. Structure-functional characterization of cytochrome P450 sterol 14alpha-demethylase (CYP51B) from Aspergillus fumigatus and molecular basis for the development of antifungal drugs. The Journal of Biological Chemistry. 2015, 290(39), 23916-23934.

Lamb DC, Warrilow AG, Rolley NJ, Parker JE, Nes WD, Smith SN, Kelly DE, Kelly SL. Azole antifungal agents to treat the human pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through inhibition of sterol 14alpha-demethylase (CYP51). Antimicrobial Agents and Chemotherapy. 2015, 59(8), 4707-4713.

2014

Morrison AM, Goldstone JV, Lamb DC, Kubota A, Lemaire B, Stegeman JJ. Identification, modeling and ligand affinity of early deuterostome CYP51s, and functional characterization of recombinant zebrafish sterol 14alpha-demethylase. Biochimia et biophysica acta. 2014, 1840(6), 1825-1836.

2013

Reid EL, Weynberg KD, Love J, Isupov MN, Littlechild JA, Wilson WH, Kelly SL, Lamb DC, Allen, MJ. Functional and structural characterisation of a viral cytochrome b5. FEBS Letters 2013, 587(22), 3633-3639.

Lamb DC, Waterman MR, Zhao B. Streptomyces cytochromes P450: applications in drug metabolism. Expert Opinion on Drug Metabolism and Toxicology. 2013, 9(10), 1279-1294.

Calabrese EC, Castellano S, Santoriello M, Sgherri C, Quartacci MF, Calucci L, Warrilow AGS, Lamb DC, Kelly SL, Milite C, Granata I, Sbardella G, Stefancich G,Maresca B, Porta A. Antifungal activity of azole compounds CPA18 and CPA109 against azole-susceptible and -resistant strains of Candida albicans. Journal of Antimicrobial Chemotherapy. 2013, 68(5), 1111-1119.

Lamb DC, Waterman MR. Unusual properties of the cytochrome P450 superfamily. Philosophical Transactions of the Royal Society B 2013, 368(16212)SI, Art. No. 20120434.

Tian Z, Cheng Q, Yoshimoto FK, Lei L, Lamb DC, Guengerich FP. Cytochrome P450 107U1 is required for sporulation and antibiotic production in Streptomyces coelicolor. Archives of Biochemistry and Biophysics 2013, 530(2), 101-107.

2012

Zhao B, Moody SC, Hider RC, LeiL, Kelly SL, Waterman MR, Lamb DC. Structural Analysis of cytochrome P450 105N1 involved in the biosynthesis of the zincophore Coelibactin. International Journal of Molecular Sciences. 2012, 13(7), 8500-8513.

Moody SC, Zhao B, Lei L, Nelson DR, Mullins JG, Waterman MR, Kelly SL, Lamb DC. Investigating conservation of the albaflavenone biosynthetic pathway and CYP170 bifunctionality in streptomycetes. FEBS Journal. 2011, 279(9), 1640-1649.

Agnew CRJ, Warrilow AGS, Burton NM, Lamb DC, Kelly SL, Brady RL. An enlarged, adaptable active site in CYP164 family P450 enzymes, the sole P450 in Mycobacterium leprae. Antimicrobial Agents and Chemotherapy.2012, 56(2), 391-402.

2010

Cheng Q, Lamb DC, Kelly SL, Lei L, Guengerich FP. Cyclization of a cellular dipentaenone by Streptomyces coelicolor cytochrome P450 154A1 without oxidation/reduction. Journal of the American Chemical Society 2010, 132(43), 15173-15175.

Warrilow AGS, Martel CM, Parker JE, Melo N, Lamb DC, Nes D, Kelly DE, Kelly SL. Azole binding properties of Candida albicans sterol 14-alpha demethylase (CaCYP51). Antimicrobial Agents and Chemotherapy. 2010, 54(10), 4235-4245.

Lamb DC, Lei L, Zhao B, Yuan H, Jackson CJ, Warrilow AGS, Skaug T, Dyson PJ, Dawson ES, Kelly SL, Hachey DL, Waterman MR. Streptomyces coelicolor A3(2) CYP102 protein, a novel fatty acid hydroxylase encoded as a heme omain without an N-terminal redox partner. Applied and Environmental Microbiology. 2010, 76(6), 1975-1980.

2009

Zhao B, Lei L, Vassylyev DG, Lin X, Cane DE, Kelly SL, Yuan H, Lamb DC, Dawson ES, Waterman MR. Crystal structure of albaflavenone monooxygenase containing a moonlighting terpene synthase active site. Journal of Biological Chemistry.2009, 284(52), 36711-36719.

Lamb DC, Lei L, Warrilow AGS, Lepesheva GI, Mullins JGL, Waterman MR, Kelly SL. The first virally encoded cytochrome P450. Journal of Virology 2009, 83 (16), 8266-8269.

Warrilow AGS, Jackson CJ, Parker JE, Marczylo TH, Kelly DE, Lamb DC, Kelly SL. Identification, characterization and azole-binding properties of Mycobacterium smegmatis CYP164A2, a homolog of ML2088, the sole cytochrome P450 gene of Mycobacterium leprae. Antimicrobial Agents and Chemotherapy 2009, 53(3) 1157-1164.

2008

Warrilow A, Ugochukwu C, Lamb D, Kelly D, Kelly S. Expression and characterization of CYP51, the ancient sterol 14-demethylase activity for cytochromes P450 (CYP), in the white-rot fungus Phanerochaete chrysosporium. Lipids 2008, 43(12) 1143-1153.

Melo NR, Moran GP, Warrilow AGS, Dudley E, Smith SN, Sullivan DJ, Lamb DC, Kelly DE, Coleman DC, Kelly SL. CYP56 (Dit2p) in Candida albicans: Characterization and investigation of its role in growth and antifungal drug susceptibility. Antimicrobial Agents and Chemotherapy 2008, 52(10) 3718-3724.

La Regina G, D'Auria FD,Tafi A, Piscitelli F, Olla S, Caporuscio F, Nencioni L, Cirilli R, La Torre F, deMelo NR, Kelly SL, Lamb DC, Artico M, Botta M, Palamara AT, Silvestri R. 1-[(3-Aryloxy-3-aryl)propyl]-2H-imidazoles, new imidazoles with potent activity against Candida albicans and dermatophytes. Synthesis, structure-activity relationship, and molecular modeling studies. Journal of Medicinal Chemistry 2008, 51(13) 3841-3855.

Jackson CJ, Lamb DC, Warrilow AGS, Parker JE, de Melo NR, Kelly DE, Kelly SL. P450s in microbial sterol biosynthesis and drug targets. Acta Chimica Slovenica 2008, 55(1) 58-62.

Zhao B, Lin X, LeiL, Lamb DC, Kelly SL, Waterman MR, Cane DE. Biosynthesis of the Sesquiterpene Antibiotic Albaflavenone in Streptomyces coelicolor A3(2). Journal of Biological Chemistry 2008, 283(13) 8183-8189.

2007

Chun YJ, ShimadaT, Sanchez-Ponce R, Martin MV, Lei L, Zhao B, Kelly SL, Waterman MR, Lamb DC, Guengerich FP. Electron transport pathway for a Streptomyces cytochrome P450 - Cytochrome P450 105D5-catalyzed fatty acid hydroxylation in Streptomyces coelicolor A3(2). Journal of Biological Chemistry 2007, 282(24) 17486-17500.

Lamb DC, Jackson CJ, Warrilow AG, Manning NJ, Kelly DE, Kelly SL. Lanosterol Biosynthesis in the Prokaryote Methylococcus capsulatus: Insight into the evolution of sterol biosynthesis. Molecular Biology and Evolution 2007, 24(8) 1714-1721.

Lamb DC, Waterman MR, Kelly SL, Guengerich FP. Cytochromes P450 and drug discovery. Current Opinion in Biotechnology 2007, 18(6) 504-512.

Zhao B, Lamb DC, Lei L, Kelly SL, Yuan H, Hachey DL, Waterman MR. Different binding modes of two flaviolin substrate molecules in cytochrome P450 158A1 (CYP158A1) compared to CYP158A2. Biochemistry 2007, 46(30) 8725-8733.

2006

Kelly SL, Lamb DC, Kelly DE. Cytochrome P450 biodiversity and biotechnology. Biochemical Society Transactions 2006, 34(6) 1159-60.

Lamb DC,Guengerich FP, Kelly SL, Waterman, MR. Exploiting Streptomyces coelicolor A3(2) P450s as a model for application in drug discovery. Expert Opinion on Drug Metabolism & Toxicology 2006, 2(1) 27-40.

Lamb DC, Kim Y, Yermalitskaya LV, Yermalitsky VN, Lepesheva GI, Kelly SL, Waterman MR, Podust LM. A second FMN binding site in yeast NADPH-cytochrome P450 reductase suggests a mechanism of electron transfer by diflavin reductases. Structure 2006, 14(1) 51-61.

RupasingheS, Schuler MA, Kagawa N, Yuan H, Lei L, Zhao B, Kelly SL, Waterman MR, Lamb,DC. The cytochrome P450 gene family CYP157 does not contain EXXR in the K-helix reducing the absolute conserved P450 residues to a single cysteine. FEBS Letters 2006, 580(27) 6338-42.

2005

Zhao B, Guengerich FP, Bellamine A, Lamb DC, Izumikawa M, Lei L, Podust LM, Sundaramoorthy M, Kalaitzis JA, Reddy LM, Kelly SL, Moore BS, Stec D, Voehler M, Falck JR, Shimada T, Waterman MR. Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2. Journal of Biological Chemistry 2005, 280(12) 11599-11607.

2004

Lei L, Waterman MR, Fulco AJ, Kelly SL, Lamb DC. Availability of specific reductases controls the temporal activity of the cytochrome P450 complement of Streptomyces coelicolor A3(2). Proceedings of the National Academy of Sciences USA 2004, 101(2) 494-499.

Podust LM, Bach H, Kim Y, Lamb DC, Arase M, Sherman DH, Kelly SL, Waterman MR. Comparison of the 1.85 A structure of CYP154A1 from Streptomyces coelicolor A3(2) with the closely related CYP154C1 and CYPs from antibiotic biosynthetic pathways. Protein Science 2004, 13(1) 255-268.

Revankar SG, Fu J, Rinaldi MG, Kelly SL, Kelly DE, Lamb DC, Keller SM, Wickes BL. Cloning and characterization of the lanosterol 14alpha-demethylase (ERG11) gene in Cryptococcus neoformans. Biochemical and Biophysical Research Communications 2004, 324(2) 719-728.

For further publications please see ResearchGate.

Affiliations
Fellow of the Royal Society of Biology
Fellow of the Royal Society of Chemistry